|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11329-ACG|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11329-ACR|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||HG11329-ANG|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||HG11329-ANR|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11329-CF|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11329-CH|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11329-CM|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11329-CY|
|人 HSPA8/HSC70 基因全長cDNA ORF(克隆載體)||HG11329-M|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11329-NF|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11329-NH|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11329-NM|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11329-NY|
|人 HSPA8/HSC70 基因全長cDNA ORF克隆 (表達載體)||HG11329-UT|
HSPA8, also known as HSC70, is a member of the heat shock protein family due to homology with other heat shock proteins. The heat shock protein 70 family is comprised by both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 belongs to the heat-shock cognate subgroup. Members of the human heat-shock protein multigene family have several highly conserved proteins with structural and functional properties in common, but vary in the extent of their inducibility in response to metabolic stress. HSPA8 is constitutively expressed and performs functions related to normal cellular processes. This protein binds to nascent polypeptides to facilitate correct protein folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. HSPA8 acts as a repressor of transcriptional activation. It inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. It also is a ATPase that works with auxilin to remove clathrin coated vesicles. In neurons, synaptojanin is also an important protein involved in vesicle uncoating.