Anti-HSP70 Antibody

Human HSP70/HSPA1A Immunofluorescence(IF) 2909
Human HSP70/HSPA1A Immunohistochemistry(IHC) 4772
Human HSP70/HSPA1A Immunohistochemistry(IHC) 4773
Human HSP70/HSPA1A Flow Cytometry (FC) 5151
Human HSP70/HSPA1A Western blot (WB) 14774
Human HSP70/HSPA1A Immunoprecipitation(IP) 14812
Human HSP70/HSPA1A Immunofluorescence(IF) 2909
Human HSP70/HSPA1A Immunohistochemistry(IHC) 4772
Human HSP70/HSPA1A Immunohistochemistry(IHC) 4773
Human HSP70/HSPA1A Flow Cytometry (FC) 5151
Human HSP70/HSPA1A Western blot (WB) 14774
Human HSP70/HSPA1A Immunoprecipitation(IP) 14812
Cat: 11660-R001

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Anti-HSP70 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-HSP70 Antibody
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human HSP70
Immunogen
Recombinant Human HSP70 / HSPA1A protein (Catalog#11660-H07B)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP70 / HSPA1A (rh HSP70 / HSPA1A; Catalog#11660-H07B; P08107; Ala2-Asp641).
Source
Monoclonal Rabbit IgG Clone #001
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-HSP70 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-HSP70 Antibody (Rabbit Monoclonal antibody) Images

Human HSP70/HSPA1A Immunofluorescence(IF) 2909
Immunofluorescence staining of Human HSPA1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSPA1A monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human HSP70/HSPA1A Immunohistochemistry(IHC) 4772
Immunochemical staining of HSPA1A in human hepatoma with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human HSP70/HSPA1A Immunohistochemistry(IHC) 4773
Immunochemical staining of HSPA1A in cynomolgus macaque testis with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human HSP70/HSPA1A Flow Cytometry (FC) 5151
Flow cytometric analysis of Human HSPA1A expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSPA1A, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Human HSP70/HSPA1A Western blot (WB) 14774

Anti-HSPA1A rabbit monoclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lane B: HCT116 Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:70 kDa

Observed band size:70 kDa

(We are unsure as to the identity of these extra bands.)

Human HSP70/HSPA1A Immunoprecipitation(IP) 14812

HSPA1A was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-HSPA1A rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HSPA1A rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 70 kDa

Observed band size: 70 kDa

Anti-HSP70 Antibody Alternative Names

Anti-HEL-S-103 Antibody;Anti-HSP70-1 Antibody;Anti-HSP70-1A Antibody;Anti-HSP70I Antibody;Anti-HSP72 Antibody;Anti-HSPA1 Antibody

HSP70 Background Information

HSPA1A is a member of the Hsp7 protein family. The 7 kilodalton heat shock proteins (Hsp7s) are a family of ubiquitously expressed heat shock proteins. HSP are abundant and conserved proteins present in all cells. Upon temperature shock or other stress stimuli, HSP are synthesized intracellularly, which may protect cells from protein denaturation or from death. Extracellularly, HSP can serve a cytokine function to initiate both innate and adaptive immunity through activation of APC. HSP serves also a chaperone function and facilitates presentation of antigen peptide to T cells. Molecular chaperones of the Hsp7 family have diverse functions in cells. They assist the folding of newly synthesized and stress-denatured proteins, as well as the import of proteins into organelles, and the dissociation of aggregated proteins. The well-conserved Hsp7 chaperones are ATP dependent: binding and hydrolysis of ATP regulates their interactions with unfolded polypeptide substrates, and ATPase cycling is necessary for their function. All cellular functions of Hsp7 chaperones use the same mechanism of ATP-driven polypeptide binding and release. 
Full Name
heat shock 70kDa protein 1A
Research Areas
References
  • Heck TG, et al. (2011) HSP70 expression: does it a novel fatigue signalling factor from immune system to the brain Cell Biochem Funct. 29 (3): 215-26.
  • Chen T, et al. (2010) Stress for maintaining memory: HSP70 as a mobile messenger for innate and adaptive immunity. Eur J Immunol. 40 (6): 1541-4.
  • Young JC. (2010) Mechanisms of the Hsp70 chaperone system. Biochem Cell Biol. 88 (2): 291-300.
    • Product Description Host Clonality Application Catalog# (PDF)
    Anti-HSP70 Antibody (FITC) Rabbit Monoclonal FCM 11660-R001-F
    Anti-HSP70 Antibody Mouse Monoclonal IHC-P 11660-MM12
    Anti-HSP70 Antibody Rabbit Polyclonal ELISA 11660-RP01
    Anti-HSP70 Antibody Mouse Monoclonal ELISA 11660-MM15
    Anti-HSP70 Antibody Rabbit Polyclonal WB,ELISA,IP 11660-T52
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