Anti-Histone H3 Antibody

Cat: 100005-MM01
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All Histone H3 Reagents
Anti-Histone H3 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Histone H3 Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human Histone H3
Immunogen
A synthetic peptide corresponding to the C-terminus of the human Histone H3.
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #1F6D7C4
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Histone H3 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1/1000-1/10000  
IHC-P 1/50-1/1000  
IP 4-10 uL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Histone H3 Antibody (Mouse Monoclonal antibody) Images
Human Histone H3 Immunohistochemistry(IHC) 4056
Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
Human Histone H3 Immunohistochemistry(IHC) 4057
Immunochemical staining of human histone H3 in human colon carcinoma with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide).
Human Histone H3 Western blot (WB) 13023
Human Histone H3 Immunoprecipitation(IP) 14030
Histone H3 Background Information

Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes.

References
  • Bhasin M, et al., 2006, 13(1): 102-12.
  • Lachner M, et al., 2001, 410(6824): 116-20.
  • Rosenfeld JA, et al., 2009, BMC Genomics. 10: 143.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Histone H3 Antibody Mouse Monoclonal ICC/IF,IF 100002-MM02
    info info
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