Anti-Histone H3 Antibody (Mouse Monoclonal antibody) General Information
Anti-Histone H3 Antibody
Reacts with: Human
Human Histone H3
A synthetic peptide corresponding to the C-terminus of the human Histone H3.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #1F6D7C4
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Histone H3 Antibody (Mouse Monoclonal antibody) Validated Applications
||4-10 uL/mg of lysate
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-Histone H3 Antibody (Mouse Monoclonal antibody) Images
Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
Immunochemical staining of human histone H3 in human colon carcinoma with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide).
Histone H3 Background Information
Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes.
Bhasin M, et al., 2006, 13(1): 102-12. Lachner M, et al., 2001, 410(6824): 116-20.Rosenfeld JA, et al., 2009, BMC Genomics. 10: 143.