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小鼠 HGF / Hepatocyte Growth Factor 蛋白

  • Mouse HGF / Hepatocyte Growth Factor Protein SDS-PAGE
產品資料評論相關產品實驗方法
小鼠 HGF 蛋白產品資訊
分子別稱:C230052L06Rik,HGF/SF,NK1,NK2,SF,SF/HGF
蛋白構建:A DNA sequence encoding the mouse HGF (Q08048-1) (Met1-Leu728) was expressed and purified.
分子種屬:Mouse
表達宿主:Human Cells
#N/A:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
小鼠 HGF 蛋白質控檢測
純度:> 95 % as determined by SDS-PAGE
生物活性:1. Immobilized mouse HGF at 10 μg/ml (100 μl/well) can bind mouse MET-Fc (Cat:50622-M02H), EC50 of mouse MET-Fc (Cat:50622-M02H) is 0.05-0.12 μg/ml.
2. Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 ng/ml.
內毒素:< 1.0 EU per μg of the protein as determined by the LAL method
穩定性:Samples are stable for up to twelve months from date of receipt at -70℃
預測N端:Gln 33
分子量:The recombinant mouse HGF comprises 696 amino acids and has a predicted molecular mass of 79.3 kDa. The apparent molecular mass of the protein is approximately 93 kDa in SDS-PAGE under reducing conditions due to glycosylation.
緩衝液:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
小鼠 HGF 蛋白使用指導
儲存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
#N/A:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
小鼠 HGF Protein SDS-PAGE
Mouse HGF / Hepatocyte Growth Factor Protein SDS-PAGE

HGF 相關信號通路

    Other HGF Recombinant Protein Products
    50038-MNAH
    HGF/Hepatocyte Growth Factor 研究背景

    Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    小鼠 HGF/Hepatocyte Growth Factor 參考資料
  • Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
  • Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
  • Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
  • Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.
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    貨號: 50038-MNAH-20
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    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”