Anti-HER3 / ERBB3 Antibody

Cat: 10201-mhA24
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Anti-HER3 / ERBB3 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-HER3 / ERBB3 Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human HER3 / ERBB3
No cross-reactivity with Mouse ErbB3 (Catalog # 51003-M08H) and Rat ErbB3 (Catalog # 80111-R08H) in ELISA assay
Immunogen
Recombinant Human HER3 / ErbB3 protein (Catalog#10201-H08H)
Preparation
It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant Human HER3 / ErbB3. The antibody was produced using recombinant antibody technology.
Source
Monoclonal mouse (varialbe region) / human (kappa / IgG1 constant) chimeric antibody Clone #mhA24
Purification
Protein A
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
Please contact us for more information.
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-HER3 / ERBB3 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Notes
Block In a functional ELISA which immobilized recombinant Human NRG1/Fc Chimera (Catalog # 11609-H01H2) at 5 μg/mL (100 µL/well) in the plate, the Mouse and Human Chimeric anti-Human ErBB3 Monoclonal Antibody (Catalog # 10201-mhA24) can block the binding of 1 μg/mL of biotinylated Human ErBB3 (Catalog # 10201-H08H) to Human NRG1/Fc Chimera, the EC50 is 1.78 μg/mL.
Neutralization The neutralization activity of Erbb3 Neutralizing Antibody is Measured by its ability to neutralize NRG1-β1/HRG1-β1 induced proliferation in the MCF-7 human breast cancer cell line. The Neutralization titer (IC50) is typically 5.05-20.2 ng/mL in the presence of 40 ng/mL Recombinant Human NRG1-β1/HRG1-β1.

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-HER3 / ERBB3 Antibody (Mouse Monoclonal antibody) Images
Human EEBB3/HER3 Blocking/Neutralization (B/N) 5595
Cell Proliferation Induced by NRG1-β1/HRG1-β1 was Neutralized by Human ErbB3/Her3 Antibody. Recombinant Human NRG1-1β1/HRG1-1β1 stimulates proliferation in the MCF-7 human breast cancer cell line. Proliferation elicited by Recombinant Human NRG1-1β1/HRG1-1β1 (40 ng/mL) is neutralized by increasing concentrations of Human ErbB3/Her3 Monoclonal Antibody (Catalog 10201-mhA24). The IC50 is typically 5.05-20.2 ng/mL.
Anti-HER3 / ERBB3 Antibody Alternative Names
Anti-c-erbB-3 Antibody;Anti-c-erbB3 Antibody;Anti-EEBB3 Antibody;Anti-ErbB-3 Antibody;Anti-erbB3-S Antibody;Anti-HER3 Antibody;Anti-LCCS2 Antibody;Anti-MDA-BF-1 Antibody;Anti-p180-ErbB3 Antibody;Anti-p45-sErbB3 Antibody;Anti-p85-sErbB3 Antibody
HER3 / ERBB3 Background Information

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
erb-b2 receptor tyrosine kinase 3
References
  • Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
  • Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
  • Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA 90043-T16
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA 90043-RP01
    Anti-HER3 / ERBB3 Antibody Mouse Monoclonal ELISA 90043-MM05
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA 80111-RP01
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA 80111-T16
    Anti-HER3 / ERBB3 Antibody Rabbit Monoclonal ELISA 51003-R028
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA 51003-RP01
    Anti-HER3 / ERBB3 Antibody Rabbit Monoclonal ELISA(Cap) 51003-R128
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA 51003-T16
    Anti-HER3 / ERBB3 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 51003-R138
    Anti-HER3 / ERBB3 Antibody Mouse Monoclonal Neutralization 10201-M908
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA 10201-T16
    Anti-HER3 / ERBB3 Antibody Rabbit Monoclonal ELISA(Det) 10201-R015
    Anti-HER3 / ERBB3 Antibody Mouse Monoclonal Neutralization 10201-mh908
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal WB,ELISA,IP 10201-RP02
    Anti-HER3 / ERBB3 Antibody (APC) Mouse Monoclonal FCM 10201-MM03-A
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA 10201-RP01
    Anti-HER3 / ERBB3 Antibody Mouse Monoclonal ELISA 10201-MM01
    Anti-HER3 / ERBB3 Antibody Rabbit Polyclonal ELISA,IHC-P 10201-T24
    Anti-HER3 / ERBB3 Antibody Mouse Monoclonal Neutralization 10201-MA24
    Anti-HER3 / ERBB3 Antibody (PE) Mouse Monoclonal FCM 10201-MM03-P
    Anti-HER3 / ERBB3 Antibody Rabbit Monoclonal ELISA 10201-R016
    Anti-HER3 / ERBB3 Antibody (FITC) Mouse Monoclonal FCM 10201-MM03-F
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