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人 HER2 / ErbB2 / CD340 ELISA Pair Set

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 ERBB2 組成成分
Capture Ab:1 mg/mL of rabbit anti-Human HER2 / ErbB2 / CD340 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 10004-R511)
檢測抗體:0.1 mg/mL of rabbit anti-Human HER2 / ErbB2 / CD340 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10004-R205)
標準品:Each vial contains 110 ng of recombinant Human HER2 / ErbB2 / CD340. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.
 ERBB2 檢測靈敏度
The minimum detectable dose of Human HER2 / ErbB2 / CD340 was determined to be approximately 24 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
 ERBB2 產品介紹
The Human HER2 / ErbB2 / CD340 ELISA Pair Set is for the quantitative determination of Human HER2 / ErbB2 / CD340.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human HER2 / ErbB2 / CD340 coated on a 96-well plate. Standards and samples are added to the wells, and any Human HER2 / ErbB2 / CD340 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human HER2 / ErbB2 / CD340 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human HER2 / ErbB2 / CD340 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
 ERBB2 儲存方法
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
 ERBB2 ELISA Pair Set Standard Curve
HER2/ERBB2 研究背景

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

 HER2/ERBB2 參考資料
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
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    貨號: SEKA10004-5
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    • Human HER2 / ErbB2 / CD340 ELISA standard curve
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