Anti-Her2 / ERBB2 Antibody

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Anti-Her2 / ERBB2 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-Her2 / ERBB2 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Rhesus
Specificity
Rhesus Her2 / ERBB2
Has cross-reactivity in ELISA with
Human ErbB2
No cross-reactivity in ELISA with
Human EGFR
Human ERBB3
Human ERBB4
Human cell lysate (293 cell line)
Immunogen
Recombinant Rhesus HER2 / ErbB2 protein (Catalog#90020-K08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rhesus HER2 / ErbB2 (Catalog#90020-K08H; XP_001090430.1; Met 1-Thr 652). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #10A7F7
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Her2 / ERBB2 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Her2 / ERBB2 Background Information

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Full Name
erb-b2 receptor tyrosine kinase 2
References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
  • Phage display as a tool for development of novel therapeutics for breast cancer
    Author
    Cabral, MJCD;
    Year
    2014
    Journal
    Thesis
    Application
    ELISA
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