Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb

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Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IP,B/N (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
H5N1 Hemagglutinin / HA
Has cross-reactivity in ELISA with
H5N1 (A/turkey/Turkey/1/2005) HA
H5N1 (A/chicken/India/NIV33487/2006) HA
H5N1 (A/Hong Kong/483/97) HA
H5N1 (A/whooper swan/mongolia/244/2005) HA
H5N1 (A/Viet nam/1194/2004) HA
H5N1 (A/goose/Guiyang/337/2006) HA
H5N1 (A/Indonesia/5/2005) HA
H5N1 (A/Cambodia/R0405050/2007) HA
H5N1 (A/duck/Hunan/795/2002) HA
H5N1 (A/Egypt/2321-NAMRU3/2007) HA
H5N1 (A/Egypt/N05056/2009) HA
H5N3 (A/duck/Hokkaido/167/2007) HA
H5N8 (A/duck/NY/191255-59/2002) HA
H5N1 (A/bar-headed goose/Qinghai/14/2008) HA
H5N1 (A/Common magpie/Hong Kong/2256/2006) HA
No cross-reactivity in ELISA with
H1N1 (A/California/04/2009) HA
H1N1 (A/Brisbane/59/2007) HA
H3N2 (A/Brisbane/10/2007) HA
Influenza B (B/Florida/4/2006) HA
Human cell lysate (293 cell line)
Immunogen
Recombinant H5N1 HA protein
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H5N1 (Avian Flu) hemagglutinin extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #9F2E3F3
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 0.1-1 μg/mL
ELISA 0.5-1 μg/mL
Notes
Block: This antibody has neutralizing activity against H5N1 (A/Anhui/1/2005) strain and H5N1 (A/bar-headed/goose/Qinghai/14/2008) strain, but no neutralizing activity against H1N1 strains through pseudotyped neutralization assay. Other H5N1 strains were not tested. The concentration of this antibody with 50% neutralization is 2 μg/ml.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin / HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
  • Hemagglutinin stalk domain from H5N1 strain as a potentially universal antigen.
    Author
    K Uranowska, J Tyborowska, A Jurek…
    Year
    2013
    Journal
    Acta biochimica polonica

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