Anti-Histone H1 Antibody

Cat: 101303-T36
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All Histone H1 Reagents
Anti-Histone H1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Histone H1 Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human Histone H1
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human H1F0 / Histone H1
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human H1F0 / Histone H1, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Histone H1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Histone H1 Antibody (Rabbit Polyclonal antibody) Images
Human H1F0/Histone H1 Immunoprecipitation(IP) 6012

Histone H1.0 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-Histone H1.0 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-Histone H1.0 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Observed band size: 35 kDa

Human H1F0/Histone H1 Western blot (WB) 6583

Anti-H1F0 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 whole cell lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:21 kDa

Observed band size:31 kDa

Anti-Histone H1 Antibody Alternative Names
Anti-CPN60 Antibody;Anti-GROEL Antibody;Anti-H10 Antibody;Anti-H1FV Antibody;Anti-HLD4 Antibody;Anti-HSP60 Antibody;Anti-HSP65 Antibody;Anti-HSPD1 Antibody;Anti-HuCHA60 Antibody;Anti-SPG13 Antibody
Histone H1 Background Information

H1 histone family, member 0 (H1F0) is a member of the H1 histone family of nuclear proteins which are a component of chromatin in eukaryotic cells. It's involved in maintaining the structure of chromatin by packing the "beads on a string" sub-structure into a high order structure. The lysine-rich H1 histone family in mammals includes eleven members. In higher eukaryotes all H1 variants have the same general structure, consisting of a central conserved globular domain and less conserved N-terminal and C-terminal tails. These tails are moderately conserved among species, but differ among variants, suggesting a specific function for each H1 variant. Studies on the role of particular subtypes at specific developmental stages in lower eukaryotes, but also in vertebrates suggest that specific subtypes of H1 participate in particular systems of gene regulation. 

Full Name
H1 histone family, member 0
References
  • Ramakrishnan V, et al. (1993) Crystal structure of globular domain of histone H5 and its implications for nucleosome binding. Nature. 362 (6417): 219-23.
  • Happel N, et al. (2009) Histone H1 and its isoforms: contribution to chromatin structure and function. Gene. 431 (1-2): 1-12.
  • Izzo A, et al. (2008) The histone H1 family: specific members, specific functions. Biol Chem. 389 (4): 333-43.
  • Product Description Host Clonality Application Catalog# (PDF)
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