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GST 標籤 抗體, 兔多抗, 抗原親和純化

    產品資料評論相關產品實驗方法
    日本血吸蟲 GST 抗體產品資訊
    免疫原:Recombinant GST protein
    Clone ID:
    Ig類型:Rabbit IgG
    濃度:
    內毒素:
    緩衝液:0.2 μm filtered solution in PBS with 5% trehalose
    抗體製備方法:Produced in rabbits immunized with purified, recombinant Glutathione S-transferase (GST). GST specific IgG was purified by GST affinity chromatography.
    Other GST Antibody Products
    Immunochemical staining of human CCNF in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
    Glutathione S-transferase/GST 研究背景

    Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

    日本血吸蟲 Glutathione S-transferase/GST 參考資料
    • Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
    • Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
    • Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
    • Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”