Anti-GST tag Antibody

Cat: 11213-MM01

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Anti-GST tag Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-GST tag Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Schistosoma japonicum
Specificity
Schistosoma japonicum GST tag
No cross-reactivity with E.coli cell lysate in ELISA.
Immunogen
Recombinant GST protein
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Glutathione S-transferase (GST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #6C10G4
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-GST tag Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1/1000-1/10000  
IP 1-4 μl/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GST tag Antibody (Mouse Monoclonal antibody) Images

Schistosoma japonicum Glutathione S-transferase/GST Immunoprecipitation(IP) 6180

Anti-GST Tag was immunoprecipitated using:

Lane A: GST-ARG1-Flag transfected 293 cell lysate (0.5mg)

Lane B: Flag-ARG1-GST transfected 293 cell lysate (0.5mg)

 

2 μg anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Schistosoma japonicum GST tag Western blot (WB) 20402

Recognize GST tag fusion protein human ERK2.

Anti-GST Tag mouse monoclonal antibody at 1:4000

Lane A: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (100ng)

Lane B: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (50ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Schistosoma japonicum GST tag Western blot (WB) 20403

Recognize GST tag fusion protein human USP7.

Anti-GST Tag mouse monoclonal antibody at 1:4000

Lane A: GST-USP7 (Recombinant protein, Cat#11681-H20B) (100ng)

Lane B: GST-USP7 (Recombinant protein, Cat#11681-H20B) (50ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Schistosoma japonicum GST tag Western blot (WB) 20404

Recognize GST tag fusion proteins which expressed by 293 cell.

Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500

Lane A: GST-FABP4-flag transfected 293 Cell Lysate (30ug)

Lane B: flag-FABP4-GST transfected 293 Cell Lysate (30ug)

Lane C: flag-AGR1-GST transfected 293 Cell Lysate (30ug)

Lane D: Negative control

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Schistosoma japonicum GST tag Western blot (WB) 20405

Recognize GST tag fusion proteins which expressed by E. coli.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250

Lane A: GST-mBID transfected E.coli cell lysate (0.5ug)

Lane B: GST-mBID transfected E.coli cell lysate (0.2ug)

Lane C: GST-mBID transfected E.coli cell lysate (0.1ug)

Lane D: GST-mBID transfected E.coli cell lysate (0.05ug)

Lane E: GST-mBID transfected E.coli cell lysate (0.02ug)

Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug)

Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug)

Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug)

Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug)

Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug)

Lane K: Empty vecter (0.5ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Schistosoma japonicum GST tag Western blot (WB) 20406

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000

Lane A: GST-mBID transfected E.coli cell lysate (0.1ug)

Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug)

Lane C: Empty vecter (0.1ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Schistosoma japonicum GST tag Western blot (WB) 20407

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000

Lane A: GST-mBID transfected E.coli cell lysate (0.2ug)

Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug)

Lane C: Empty vecter (0.2ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Product Description Host Clonality Application Catalog# (PDF)
Anti-GST tag Antibody Rabbit Polyclonal ELISA 11213-RP04
Anti-GST tag Antibody Rabbit Polyclonal ELISA 11213-RP01
Anti-GST tag Antibody Mouse Monoclonal ELISA 11213-MM02
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