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人 GRIA1 基因全長cDNA ORF(克隆載體)

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 GRIA1 cDNA Clone產品資訊
註冊碼:BC111734
cDNA基因長度:2721bp
cDNA基因描述:Full length Clone DNA of Homo sapiens glutamate receptor, ionotropic, AMPA 1.
基因別名:GLUH1, GLUR1, GLURA, GluA1, HBGR1
分子種屬:Human
載體:pGEM-T Vector
Plasmid:pGEM-GRIA1
限制性酶切位點:
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
( We provide with GRIA1 qPCR primers for gene expression analysis, HP104383 )
Promoter:
Application:
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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