Anti-GFP Antibody

Cat: 13105-MM05

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Anti-GFP Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-GFP Antibody
Validated applications
ELISA,ICC/IF,IF,IP
Species reactivity
Reacts with: Aequorea victoria
Specificity
Aequorea victoria GFP
No cross-reactivity in ELISA with
E.coli cell lysate
Immunogen
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser 2-Lys 238). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-GFP Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution Notes
ELISA 0.5-1 μg/mL  
ICC-IF 10-25 μg/mL  
IP 1-4 μg/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GFP Antibody (Mouse Monoclonal antibody) Images

Aequorea victoria Green fluorescent protein / GFP Immunofluorescence(IF) 3042
Immunofluorescence staining of GFP protein in CHO cells, transfected with GFP. Cells (left: GFP, middle: antibody, right: merge) were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-GFP monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue).
Aequorea victoria Green fluorescent protein / GFP Immunoprecipitation(IP) 8157

Anti-GFP Antibody Alternative Names

Anti-GFP Antibody

GFP Background Information

The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

References
  • Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637.
  • Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.
  • Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067.
  • Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028.
  • Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.
  • Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
  • Product Description Host Clonality Application Catalog# (PDF)
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