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GFP 抗體, 鼠單抗

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Aequorea victoria GFP 抗體產品資訊
免疫原:Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Clone ID:05
Ig類型:Mouse IgG1
濃度:
內毒素:
緩衝液:0.2 μm filtered solution in PBS with 5% trehalose
抗體製備方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser 2-Lys 238). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Aequorea victoria GFP 抗體使用指導
特異性:Aequorea victoria GFP
No cross-reactivity in ELISA with
E.coli cell lysate
推薦應用:ELISA, ICC/IF, IF, IP

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is approximately 0.16 ng/well.

ICC/IF: 10-25 μg/mL

IP: 1-4 μg/mg of lysate

儲存方法:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Aequorea victoria GFP Antibody IF Application Image
GFP Antibody, Mouse Mab, Immunofluorescence
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Caption:
Immunofluorescence staining of GFP protein in CHO cells, transfected with GFP. Cells (left: GFP, middle: antibody, right: merge) were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-GFP monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue).
Aequorea victoria GFP Antibody IP Application Image
Other GFP Antibody Products
Green fluorescent protein / GFP 研究背景

The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

Aequorea victoria Green fluorescent protein / GFP 參考資料
  • Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637.
  • Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.
  • Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067.
  • Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028.
  • Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.
  • Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
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    Size / Price
    貨號: 13105-MM05-50
    目錄價: 
    單價:      (You Save: )
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”