Anti-GFP Antibody

Cat: 13105-R208
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Anti-GFP Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-GFP Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Aequorea victoria
Specificity
Aequorea victoria GFP
Immunogen
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser2-Lys238).
Source
Monoclonal Rabbit IgG Clone #208
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GFP Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1/1000-1/5000  
ELISA 1/10000-1/20000  
IP 1-4 μl/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GFP Antibody (Rabbit Monoclonal antibody) Images
Aequorea victoria Green fluorescent protein / GFP Immunoprecipitation(IP) 8158
Aequorea victoria Green fluorescent protein / GFP Western blot (WB) 13529
Anti-GFP Antibody Alternative Names
Anti-GFP Antibody
GFP Background Information

The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

References
  • Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637.
  • Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.
  • Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067.
  • Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028.
  • Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.
  • Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-GFP Antibody Rabbit Polyclonal ELISA 13105-RP01
    Anti-GFP Antibody Rabbit Polyclonal ELISA 13105-T16
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