Anti-GAPDH Antibody

Cat: 100242-MM05
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Anti-GAPDH Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-GAPDH Antibody
Validated applications
WB
Species reactivity
Reacts with: Human
Specificity
Human GAPDH
Immunogen
A synthetic peptide corresponding to the center region of the human GAPDH
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human GAPDH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GAPDH Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GAPDH Antibody (Mouse Monoclonal antibody) Images
Human GAPDH Western blot (WB) 6437

Anti-GAPDH mouse monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lane C: MCF7 Whole Cell Lysate

Lane D: MCF7 whole cell lysate

Lane E: PC-3 whole cell lysate

Lane F: Daudi whole cell lysate

Lane G: A549 whole cell lysate

Lane H: Rat heart tissue lysate

Lane I: Rat liver tissue lysate

Lane J: Rat spleen tissue lysate

Lane K: Rat lung tissue lysate

Lane L: Rat kidney tissue lysate

Lane M: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size: kDa

Observed band size:38 kDa

Anti-GAPDH Antibody Alternative Names
Anti-G3PD Antibody;Anti-GAPD Antibody;Anti-HEL-S-162eP Antibody
GAPDH Background Information

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Full Name
glyceraldehyde-3-phosphate dehydrogenase
References
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
  • Product Description Host Clonality Application Catalog# (PDF)
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