Anti-GCSF Receptor / G-CSFR Antibody

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Anti-GCSF Receptor / G-CSFR Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-GCSF Receptor / G-CSFR Antibody
Validated applications
ELISA(Cap),ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human GCSF Receptor / G-CSFR
Immunogen
Recombinant Human G-CSFR / CD114 protein (Catalog#10218-HCCH)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human G-CSFR / CD114 (rh G-CSFR / CD114; Catalog#10218-HCCH; NP_000751.1; Met1-Pro621). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #06
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GCSF Receptor / G-CSFR Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ICC-IF 1:20-1:100
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Human G-CSF R / CD114 / CSF3R in ELISA pair set (Catalog: # SEK10218). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10218-MM01).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GCSF Receptor / G-CSFR Antibody (Mouse Monoclonal antibody) Images

Confocal immunofluorescence analysis of Human GCSFR in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human GCSFR monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm and cytomembrane.

Anti-GCSF Receptor / G-CSFR Antibody: Alternative Names

Anti-CD114 Antibody; Anti-CSF3R Antibody; Anti-G-CSF R Antibody; Anti-GCSFR Antibody

GCSF Receptor / G-CSFR Background Information

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.
Full Name
colony stimulating factor 3 receptor (granulocyte)
References
  • Kasper B, et al. (1999) Association of src-kinase Lyn and non-src-kinase Syk with the granulocyte colony-stimulating factor receptor (G-CSFR) is not abrogated in neutrophils from severe congenital neutropenia patients with point mutations in the G-CSFR mRNA. Int J Hematol. 70(4): 241-7.
  • Hollenstein U, et al. (2000) Endotoxin down-modulates granulocyte colony-stimulating factor receptor (CD114) on human neutrophils. J Infect Dis. 182(1): 343-6.
  • Kindwall-Keller TL, et al. (2008) Role of the proteasome in modulating native G-CSFR expression. Cytokine. 43(2): 114-23.
  • Beel K, et al. (2009) G-CSF receptor (CSF3R) mutations in X-linked neutropenia evolving to acute myeloid leukemia or myelodysplasia. Haematologica. 94(10): 1449-52.
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