All EphB2 reagents are produced in house and quality controlled, including 5 EphB2 Antibody, 24 EphB2 Gene, 6 EphB2 Lysate, 6 EphB2 Protein, 2 EphB2 qPCR. All EphB2 reagents are ready to use.
Recombinant EphB2 proteins are expressed by HEK293 Cells, Baculovirus-Insect Cells with fusion tags as C-His, C-human IgG1-Fc & His, N-GST & His, C-cleavage, C-human IgG1-Fc.
EphB2antibodies are validated with different applications, which are ELISA, IHC-P, FCM.
EphB2cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each EphB2 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Expression host: Baculovirus-Insect Cells
Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.