Anti-EGFR Antibody (Rabbit Monoclonal antibody) General Information
Reacts with: Human
No cross-reactivity in ELISA with
Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met 1-Ser 645).
Monoclonal Rabbit IgG Clone #043
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-EGFR Antibody (Rabbit Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-EGFR Antibody (Rabbit Monoclonal antibody) Images
Immunofluorescence staining of EGFR in monkey skin with rabbit monoclonal antibody (1:50, frozen section). The image showing membrane staining of squamous epithelium cell.
Immunochemical staining of human EGFR in human uterine cervix cancer with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to uterine gland.
Immunochemical staining of human EGFR in human rectal cancer with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to rectal gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Immunochemical staining of human EGFR in human placenta with rabbit monoclonal antibody (1:200,formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.
Anti-EGFR Antibody Alternative Names
Anti-ERBB Antibody;Anti-ERBB1 Antibody;Anti-HER1 Antibody;Anti-mENA Antibody;Anti-NISBD2 Antibody;Anti-PIG61 Antibody
EGFR Background Information
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
epidermal growth factor receptor
Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25. Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48. Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.