Anti-EGFR Antibody

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Anti-EGFR Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-EGFR Antibody
Validated applications
WB,ELISA,ELISA(Det),ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human EGFR
No cross-reactivity in ELISA with
Human ErbB2 / HER2
Human ErbB3 / HER3
Immunogen
Recombinant Human EGFR Protein(Catalog#10001-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR (rh EGFR; Catalog#10001-H08H; Met 1-Ser 645; NP_005219) .
Source
Monoclonal Rabbit IgG Clone #021
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-EGFR Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
ICC-IF 1:100-1:500
ELISA(Det) 1:5000-1:50000
Notes
ELISA(Det): This antibody will detect Human EGFR / HER1 in ELISA pair set (Catalog: # SEK10001). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10001-RE11).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EGFR Antibody (Rabbit Monoclonal antibody) Images

Anti-EGFR rabbit monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:180 kDa

Observed band size:180 kDa

Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human EGFR monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Anti-EGFR Antibody: Alternative Names

Anti-ERBB Antibody; Anti-ERBB1 Antibody; Anti-HER1 Antibody; Anti-mENA Antibody; Anti-NISBD2 Antibody; Anti-PIG61 Antibody

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.

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