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EGFR / HER1 / ErbB1 抗體, 鼠單抗

產品資料評論相關產品實驗方法
Human EGFR 抗體產品資訊
免疫原:Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
Clone ID:08
Ig類型:Mouse IgG1
濃度:
內毒素:
緩衝液:0.2 μm filtered solution in PBS with 5% trehalose
抗體製備方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human EGFR 抗體使用指導
特異性:Human EGFR / HER1 / ErbB1
No cross-reactivity in ELISA with
Human ErbB2
Human ErbB3
Human ErbB4
推薦應用:ICC/IF, IF, FCM

FCM: 0.5-2 μg/Test

ICC/IF: 5-20 μg/mL

儲存方法:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human EGFR Antibody IHC Application Image
EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Immunofluorescence
[點擊查看大圖]
Caption:
Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human EGFR monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Human EGFR Antibody FC Application Image
EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Flow cytometric analysis
[點擊查看大圖]
Caption:
Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other EGFR Antibody Products
EGFR/HER1 研究背景

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Human EGFR/HER1 參考資料
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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    Size / Price
    貨號: 10001-MM08-50
    目錄價: 
    單價:      (You Save: )
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”