Anti-EGFR Antibody

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Anti-EGFR Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-EGFR Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Rat
Specificity
Rat EGFR
Immunogen
Recombinant Rat EGFR / HER1 / ErbB1 protein (Catalog#80100-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat EGFR / HER1 / ErbB1 (Catalog#80100-R08H; E7CXR8; Met1-Ser646). EGFR / HER1 / ErbB1 specific IgG was purified by Rat EGFR / HER1 / ErbB1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-EGFR Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EGFR Antibody (Rabbit Polyclonal antibody) Images

Anti-EGFR rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:170 kDa

Observed band size:170 kDa

(We are unsure as to the identity of these extra bands.)

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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