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大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤

產品資料評論相關產品實驗方法
E. coli FABI cDNA Clone產品資訊
註冊碼:
cDNA基因長度:789bp
cDNA基因描述:Full length Clone DNA of Escherichia coli enoyl-[acyl-carrier-protein] reductase, NADH-dependent with N terminal HA tag.
基因別名:envM
分子種屬:E. coli
載體:pCMV3-N-HA
Plasmid:
限制性酶切位點:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤 on other vectors
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤EG30001-ACGNT$8150
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤EG30001-ACRNT$8150
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤EG30001-ANGNT$8150
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤EG30001-ANRNT$8150
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤EG30001-CFNT$7060
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), C-His 標籤EG30001-CHNT$7060
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤EG30001-CMNT$7060
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤EG30001-CYNT$7060
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF(克隆載體)EG30001-MNT$2720
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤EG30001-NFNT$7060
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), N-His 標籤EG30001-NHNT$7060
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤EG30001-NMNT$7060
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤EG30001-NYNT$7060
大腸桿菌 Enoyl-ACP Reductase/FABI 基因全長cDNA ORF克隆 (表達載體)EG30001-UTNT$7060
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研究背景

Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to the short-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.

參考資料
  • Miller, WH. et al., 2002, J Med Chem  45 (15):3246-56. 
  • Kitagawa, H. et al., 2007, Bioorg Med Chem  15 (2):1106-16.
  • Zheng, C.J. et al., 2007, Biochem Biophys Res Commun 362 (4):1107-12.
  • Lu,H. et al., 2008, Acc Chem Res 41 (1):11-20.
  • Zheng,CJ. et al., 2009, J Antimicrob Chemother 63 (5):949-53.
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