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Dengue virus DENV-2 (strain New Guinea C) NS4B ORF mammalian expression plasmid, C-HA 標籤

    產品資料評論相關產品實驗方法
    DENV DENV-NS4B cDNA Clone產品資訊
    註冊碼:AF038403.1
    cDNA基因長度:744bp
    cDNA基因描述:Full length Clone DNA of DENV-2 (strain New Guinea C) NS4B with C terminal HA tag.
    基因別名:DENV-NS4B
    分子種屬:DENV
    載體:pCMV3-C-HA
    Plasmid:
    限制性酶切位點:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列資訊:Identical with the Gene Bank Ref. ID sequence AF038403.1 (6826..7569), corresponding to amino acid sequence AAC59275.1 (aa 2244-2491).
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    Dengue virus DENV-2 (strain New Guinea C) NS4B ORF mammalian expression plasmid, C-HA 標籤 on other vectors
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    貨號: VG40266-CY
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