|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||CG90001-ACG|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||CG90001-ACR|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||CG90001-ANG|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||CG90001-ANR|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||CG90001-CF|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||CG90001-CH|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||CG90001-CM|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||CG90001-CY|
|食蟹猴 p53 基因全長cDNA ORF(克隆載體)||CG90001-G|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||CG90001-NF|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||CG90001-NH|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||CG90001-NM|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||CG90001-NY|
|食蟹猴 p53 基因全長cDNA ORF克隆 (表達載體)||CG90001-UT|
p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitn ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.