|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||CG90003-ACG|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||CG90003-ACR|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||CG90003-CF|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||CG90003-CH|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||CG90003-CM|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||CG90003-CY|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF(克隆載體)||CG90003-G|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||CG90003-NF|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||CG90003-NH|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||CG90003-NM|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||CG90003-NY|
|恒河猴 SFTPD/SP-D 基因全長cDNA ORF克隆 (表達載體)||CG90003-UT|
Surfactant pulmonary-associated protein D, also known as SFTPD and SP-D, is a member of the collectin family of C-type lectins that is synthesized in many tissues including respiratory epithelial cells in the lung, and contains one C-type lectin domain and one collagen-like domain. The polymorphic variation in the N-terminal domain of the SP-D molecule influences oligomerization, function, and the concentration of the molecule in serum. SFTPD is produced primarily by alveolar type II cells and nonciliated bronchiolar cells in the lung and is constitutively secreted into the alveoli where it influences surfactant homeostasis, effector cell functions, and host defense. It is upregulated in a variety of inflammatory and infectious conditions including Pneumocystis pneumonia and asthma. SFTPD is humoral molecules of the innate immune system, and is considered a functional candidate in chronic periodontitis. Besides it is involved in the development of acute and chronic inflammation of the lung. Several human lung diseases are characterized by decreased levels of bronchoalveolar SFTPD. Thus, recombinant SFTPD has been proposed as a therapeutical option for cystic fibrosis, neonatal lung disease and smoking-induced emphysema. Furthermore, SFTPD serum levels can be used as disease activity markers for interstitial lung diseases.