|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||CG90052-ACG|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||CG90052-ACR|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||CG90052-CF|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||CG90052-CH|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||CG90052-CM|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||CG90052-CY|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF(克隆載體)||CG90052-G|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||CG90052-NF|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||CG90052-NH|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||CG90052-NM|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||CG90052-NY|
|恒河猴 CD20/MS4A1 基因全長cDNA ORF克隆 (表達載體)||CG90052-UT|
CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed on all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.