|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||CG90177-ACG|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||CG90177-ACR|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||CG90177-CF|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||CG90177-CH|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||CG90177-CM|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||CG90177-CY|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF(克隆載體)||CG90177-G|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||CG90177-NF|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||CG90177-NH|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||CG90177-NM|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||CG90177-NY|
|恒河猴 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體)||CG90177-UT|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.