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食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤

產品資料評論相關產品實驗方法
Cynomolgus GOT1 cDNA Clone產品資訊
註冊碼:NM_001284930.1
cDNA基因長度:1242bp
cDNA基因描述:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Aspartate aminotransferase, cytoplasmic with N terminal Myc tag.
基因別名:cCAT, cAspAT
分子種屬:Cynomolgus
載體:pCMV3-N-Myc
Plasmid:
限制性酶切位點:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤 on other vectors
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤CG90649-ACGNT$8150
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤CG90649-ACRNT$8150
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤CG90649-ANGNT$8150
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤CG90649-ANRNT$8150
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤CG90649-CFNT$7060
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤CG90649-CHNT$7060
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤CG90649-CMNT$7060
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤CG90649-CYNT$7060
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF(克隆載體)CG90649-GNT$2720
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤CG90649-NFNT$7060
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤CG90649-NHNT$7060
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤CG90649-NMNT$7060
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤CG90649-NYNT$7060
食蟹猴 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體)CG90649-UTNT$7060
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研究背景

Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.

參考資料
  • Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5.
  • Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9.
  • Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9.
  • Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.
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    貨號: CG90649-NM
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