|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus CD52 ORF mammalian expression plasmid, C-GFPSpark tag||CG90187-ACG|
|Rhesus CD52 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90187-ACR|
|Rhesus CD52 ORF mammalian expression plasmid, C-Flag tag||CG90187-CF|
|Rhesus CD52 ORF mammalian expression plasmid, C-His tag||CG90187-CH|
|Rhesus CD52 ORF mammalian expression plasmid, C-Myc tag||CG90187-CM|
|Rhesus CD52 ORF mammalian expression plasmid, C-HA tag||CG90187-CY|
|Rhesus CD52 Gene cDNA clone plasmid||CG90187-G|
|Rhesus CD52 ORF mammalian expression plasmid, N-Flag tag||CG90187-NF|
|Rhesus CD52 ORF mammalian expression plasmid, N-His tag||CG90187-NH|
|Rhesus CD52 ORF mammalian expression plasmid, N-Myc tag||CG90187-NM|
|Rhesus CD52 ORF mammalian expression plasmid, N-HA tag||CG90187-NY|
|Rhesus CD52 natural ORF mammalian expression plasmid||CG90187-UT|
CD52 / CDW52 is a small glycosylphosphatidylinositol (GPI) anchored glycoprotein. It has a mature peptide comprising only 12 amino acids and is abundantly expressed on human lymphocytes. From the clinical point of view this protein is an important target for therapeutic interventions aimed at leukocyte depletion in hematological malignancies and post-transplant immunosuppression. CD52 / CDW52 may play a role in carrying and orienting carbohydrate. It is an unusually good target for complement-mediated cell lysis.