|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||CG90049-ACG|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||CG90049-ACR|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||CG90049-CF|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||CG90049-CH|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||CG90049-CM|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||CG90049-CY|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF(克隆載體)||CG90049-G|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||CG90049-G-F|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||CG90049-NF|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||CG90049-NH|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||CG90049-NM|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||CG90049-NY|
|恒河猴 CD27/TNFRSF7 基因全長cDNA ORF克隆 (表達載體)||CG90049-UT|
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.