|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||DG70035-ACG|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||DG70035-ACR|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||DG70035-CF|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||DG70035-CH|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||DG70035-CM|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||DG70035-CY|
|狗 TrkB/NTRK2 基因全長cDNA ORF(克隆載體)||DG70035-G|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||DG70035-NF|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||DG70035-NH|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||DG70035-NM|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||DG70035-NY|
|狗 TrkB/NTRK2 基因全長cDNA ORF克隆 (表達載體)||DG70035-UT|
TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.