SCF cDNA ORF Clone, Canine, C-DDK (Flag®) tag

Cat: DG70010-CF

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All SCF Reagents

CRO Services

Product recommended


All SCF Reagents

SCF cDNA ORF Clone, Canine, C-DDK (Flag®) tag General Information

Gene

Species
Canine
NCBI Ref Seq
RefSeq ORF Size
825 bp
Description
Full length Clone DNA of Canine KIT ligand with C terminal Flag tag.

Plasmid

Promoter
Enhanced CMV promoter
Vector
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression

Storage & Shipping

Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SCF Background Information

Similar to Kit ligand precursor (C-kit ligand) , also known as Stem cell factor (SCF), Mast cell growth factor (MGF) or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage- colony- stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.

Full Name
KIT ligand
References
  • McNiece IK, et al. (1995) Stem cell factor. J Leukoc Biol. 58(1): 14-22.
  • Besmer P, et al. (1993) The kit-ligand (steel factor) and its receptor c-kit/W: pleiotropic roles in gametogenesis and melanogenesis. Dev Suppl. 1993:125-37.
  • Mekori YA, et al. (1993) IL-3-dependent murine mast cells undergo apoptosis on removal of IL-3. Prevention of apoptosis by c-kit ligand. J Immunol. 151(7): 3775-84.
  • SCF cDNA ORF Clone, Canine, Related Products

    Product Name Catalog#(PDF)
    SCF cDNA ORF Clone in Cloning Vector, Canine DG70010-M
    SCF cDNA ORF Clone, Canine, untagged DG70010-UT
    SCF Lentiviral cDNA ORF Clone, Canine, C-GFPSpark® tag DG70010-ACGLN
    SCF cDNA ORF Clone, Canine, C-GFPSpark® tag DG70010-ACG
    SCF cDNA ORF Clone, Canine, C-OFPSpark® tag DG70010-ACR
    SCF cDNA ORF Clone, Canine, N-DDK (Flag®) tag DG70010-NF
    SCF cDNA ORF Clone, Canine, C-His tag DG70010-CH
    SCF cDNA ORF Clone, Canine, N-His tag DG70010-NH
    SCF cDNA ORF Clone, Canine, C-HA tag DG70010-CY
    SCF cDNA ORF Clone, Canine, N-HA tag DG70010-NY
    SCF cDNA ORF Clone, Canine, C-Myc tag DG70010-CM
    SCF cDNA ORF Clone, Canine, N-Myc tag DG70010-NM

    SCF cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    SCF cDNA ORF Clone in Cloning Vector, Rat RG82498-U
    SCF Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG82498-ACGLN

    SCF cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    SCF cDNA ORF Clone in Cloning Vector, Mouse MG50487-M
    SCF cDNA ORF Clone, Mouse, untagged MG50487-UT
    SCF cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50487-ACG
    SCF Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50487-ACGLN
    SCF cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50487-ACR
    SCF cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50487-CF
    SCF cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50487-NF
    SCF cDNA ORF Clone, Mouse, C-His tag MG50487-CH
    SCF cDNA ORF Clone, Mouse, N-His tag MG50487-NH
    SCF cDNA ORF Clone, Mouse, C-HA tag MG50487-CY
    SCF cDNA ORF Clone, Mouse, N-HA tag MG50487-NY
    SCF cDNA ORF Clone, Mouse, C-Myc tag MG50487-CM
    SCF cDNA ORF Clone, Mouse, N-Myc tag MG50487-NM
    SCF qPCR Primer Pairs, Mouse MP200481

    SCF cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    SCF cDNA ORF Clone in Cloning Vector, Human HG10451-M
    SCF cDNA ORF Clone, Human, untagged HG10451-UT
    SCF Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10451-ACGLN
    SCF cDNA ORF Clone, Human, C-GFPSpark® tag HG10451-ACG
    SCF cDNA ORF Clone, Human, C-OFPSpark® tag HG10451-ACR
    SCF cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10451-CF
    SCF cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10451-NF
    SCF cDNA ORF Clone, Human, C-His tag HG10451-CH
    SCF cDNA ORF Clone, Human, N-His tag HG10451-NH
    SCF cDNA ORF Clone, Human, C-HA tag HG10451-CY
    SCF cDNA ORF Clone, Human, N-HA tag HG10451-NY
    SCF cDNA ORF Clone, Human, C-Myc tag HG10451-CM
    SCF cDNA ORF Clone, Human, N-Myc tag HG10451-NM
    SCF qPCR Primer Pairs, Human HP100480
    info info
    添加购物车成功! 添加购物车失败!