IL-10 cDNA ORF Clone, Canine, C-DDK (Flag®) tag

Cat: DG70022-CF
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IL-10 cDNA ORF Clone, Canine, C-DDK (Flag®) tag General Information
Gene
Species
Canine
NCBI Ref Seq
RefSeq ORF Size
546 bp
Description
Full length Clone DNA of Canine interleukin 10 with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

IL-10 Background Information

IL-10 is a anti-inflammatory cytokine which belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
interleukin 10
Research Areas
References
  • Arimoto T, et al. (2007) Interleukin-10 protects against inflammation-mediated degeneration of dopaminergic neurons in substantia nigra. Neurobiol Aging. 28(6):894-906.
  • Han X, et al. (2010) Effect of cobalt protoporphyrin on hyperexpression of heme oxygenase-1 and secretion of IL-10 in rat bone marrow mesenchymal stem cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 18(5):1297-301.
  • Cui QQ, et al. (2011) Expression of RhoA in the lung tissue of acute lung injury rats and the influence of RhoA on the expression of IL-8 and IL-10. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 77(7): 1436-41.
  • IL-10 cDNA ORF Clone, Canine, Related Products

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    IL-10 cDNA ORF Clone, Canine, C-His tag DG70022-CH
    IL-10 cDNA ORF Clone, Canine, N-His tag DG70022-NH
    IL-10 cDNA ORF Clone, Canine, C-HA tag DG70022-CY
    IL-10 cDNA ORF Clone, Canine, N-HA tag DG70022-NY
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    IL-10 cDNA ORF Clone, Canine, N-Myc tag DG70022-NM

    IL-10 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
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    IL-10 cDNA ORF Clone, Mouse, Related Products

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    IL-10 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50245-CF
    IL-10 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50245-NF
    IL-10 cDNA ORF Clone, Mouse, C-His tag MG50245-CH
    IL-10 cDNA ORF Clone, Mouse, N-His tag MG50245-NH
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    IL-10 cDNA ORF Clone, Human, Related Products

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    IL-10 cDNA ORF Clone, Human, C-HA tag HG10947-CY
    IL-10 cDNA ORF Clone, Human, N-HA tag HG10947-NY
    IL-10 cDNA ORF Clone, Human, C-Myc tag HG10947-CM
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