|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||DG70037-ACG|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||DG70037-ACR|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||DG70037-CF|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||DG70037-CH|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||DG70037-CM|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||DG70037-CY|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF(克隆載體)||DG70037-G|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||DG70037-NF|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||DG70037-NH|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||DG70037-NM|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||DG70037-NY|
|狗 IGF1/IGF‑I/IGF-1 基因全長cDNA ORF克隆 (表達載體)||DG70037-UT|
IGF I, also known as mechano growth factor, somatomedin-C, IGF-I and IGF1, is a secreted protein which belongs to the?insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ) and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.