|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||DG70029-ACG|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||DG70029-ACR|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||DG70029-CF|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||DG70029-CH|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||DG70029-CM|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||DG70029-CY|
|狗 BDNF/ANON2 基因全長cDNA ORF(克隆載體)||DG70029-G|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||DG70029-NF|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||DG70029-NH|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||DG70029-NM|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||DG70029-NY|
|狗 BDNF/ANON2 基因全長cDNA ORF克隆 (表達載體)||DG70029-UT|
BDNF is a member of the nerve growth factor family. It is highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. It also can be detected in heart, lung, skeletal muscle, testis, prostate and placenta. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. It participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. It functions as the major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.