All Calreticulin reagents are produced in house and quality controlled, including 2 Calreticulin Antibody, 39 Calreticulin Gene, 1 Calreticulin IPKit, 2 Calreticulin Lysate, 2 Calreticulin Protein, 3 Calreticulin qPCR. All Calreticulin reagents are ready to use.
Recombinant Calreticulin proteins are expressed by HEK293 Cells with fusion tags as C-human IgG1-Fc, C-His.
Calreticulinantibodies are validated with different applications, which are WB, ELISA, IHC-P, IP.
CalreticulincDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each Calreticulin of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.