pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80712-ACG|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80712-ACR|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80712-CF|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80712-CH|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80712-CM|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80712-CY|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80712-NF|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80712-NH|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80712-NM|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80712-NY|
|大鼠 CTHRC1 基因全長cDNA ORF(克隆載體)||RG80712-U|
|大鼠 CTHRC1 基因全長cDNA ORF克隆 (表達載體)||RG80712-UT|
Collagen triple helix repeat-containing protein 1, also known as Protein NMTC1, and CTHRC1, is a secreted protein that is glycosylated and highly conserved from lower chordates to mammals. CTHRC1 expression was not detectable in normal arteries. However, it is transiently expressed in the arterial wall in response to injury where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration. A short collagen motif with 12 Gly-X-Y repeats appears to be responsible for trimerization of the CTHRC1 protein and this renders the molecule susceptible to cleavage by collagenase. CTHRC1 overexpression caused a dramatic reduction in collagen type I mRNA and protein levels. Currently available data indicate that Cthrc1 expression in vascular cells regulates transforming growth factor beta responsiveness, thereby impacting transforming growth factor beta target genes, including collagens. Additionally, CTHRC1 increases bone mass as a positive regulator of osteoblastic bone formation and offers an anabolic approach for the treatment of osteoporosis.