CHEK1 Protein, Mouse, Recombinant (His & GST Tag)

Cat: 50248-M20B

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CHEK1 Protein, Mouse, Recombinant (His & GST Tag) Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Kinase activity untested
Protein Construction
A DNA sequence encoding the mouse CHEK1 (O35280-1) (Met1-Thr476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
Mouse
Predicted N Terminal
Met
Molecule Mass
The recombinant mouse CHEK1/GST chimera consists of 713 amino acids and has a calculated molecular mass of 82.2 kDa. The recombinant protein migrates as an approximately 78 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.5, 3mM DTT, 10% gly
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CHEK1 Protein, Mouse, Recombinant (His & GST Tag) images

Mouse Chk1 Protein 11516

CHEK1 Protein, Mouse, Recombinant (His & GST Tag) Alternative Names

C85740 Protein, Mouse;Chk1 Protein, Mouse;rad27 Protein, Mouse

CHEK1 Background Information

CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Full Name
checkpoint kinase 1
References
  • Chen P, et al. (2000) The 1.7 a crystal structure of human cell cycle checkpoint kinase CHK1 / CHEK1: Implications for CHK1 / CHEK1 regulation. Cell. 100 (6): 681-92.
  • Sanchez Y, et al. (1997) Conservation of the CHK1 / CHEK1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science. 277 (5331): 1497-501.
  • Flaggs G, et al. (1998) Atm-dependent interactions of a mammalian CHK1 / CHEK1 homolog with meiotic chromosomes. Curr Biol. 7 (12): 977-86.
  • Chini CC, et al. (2005) Claspin, a regulator of CHK1 / CHEK1 in DNA replication stress pathway. DNA Repair. 3 (8-9): 1033-7.
  • Other CHEK1 Proteins

    Human CHEK1 Proteins

    Product Name Cat.No.
    CHEK1 Protein, Human, Recombinant (GST Tag) 10539-H09B
    info info
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