The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG15760-ACG|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG15760-ACR|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG15760-CF|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG15760-CH|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG15760-CM|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG15760-CY|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG15760-NF|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG15760-NH|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG15760-NM|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG15760-NY|
|人 CD99L2 基因全長cDNA ORF克隆 (表達載體)||HG15760-UT|
Mouse CD99 antigen-like protein 2, also known as MIC2-like protein 1, CD99L2 and MIC2L1, is a single-pass type I membrane protein which belongs to the CD99 family. CD99L2 is expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression of CD99L2 is seen in thymus. It is also expressed in E18 uterus and placenta. CD99 and CD99L2 were required for leukocyte extravasation in the cremaster after stimulation with tumor necrosis factor-alpha, where the need for PECAM-1 is known to be bypassed. CD99 and CD99L2 act independently of PECAM-1 in leukocyte extravasation and cooperate in an independent way to help neutrophils overcome the endothelial basement membrane. CD99L2 may function as a homophilic adhesion molecule. It functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. CD99L2 does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis.