All CD73 reagents are produced in house and quality controlled, including 15 CD73 Antibody, 52 CD73 Gene, 1 CD73 IPKit, 4 CD73 Lysate, 4 CD73 Protein, 3 CD73 qPCR. All CD73 reagents are ready to use.
Recombinant CD73 proteins are expressed by HEK293 Cells with fusion tags as C-His.
CD73antibodies are validated with different applications, which are WB, IHC-P, ELISA, IP, FCM, ICC/IF, IF.
CD73cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each CD73 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.