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CD3e / CD3 epsilon 抗體 (FITC), 兔單抗

  • CD3e / CD3 epsilon Antibody, Rabbit MAb, Flow Cytometry
  • Human CD3e/CD3 epsilon Flow Cytometry (FC) 5262
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 CD3E 抗體產品資訊
免疫原:Recombinant Human CD3e / CD3 epsilon Protein (Catalog#10977-H08H)
Clone ID:301
Ig類型:Rabbit IgG
濃度:5 μl/Test, 0.1 mg/ml
內毒素:
緩衝液:Aqueous solution containing 0.5% BSA and 0.09% sodium azide
抗體製備方法:This antibody was obtained from a rabbit immunized with purified, recombinant Human CD3e / CD3 epsilon (rh CD3e / CD3 epsilon; Catalog#10977-H08H; NP_000724.1; Met1-Asp126) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
 CD3E Antibody FC Application Image
CD3e / CD3 epsilon Antibody, Rabbit MAb, Flow Cytometry
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Caption:
Flow cytometric analysis of Human CD3E expression on human whole blood lymphocytes. Cells were stained with FITC-conjugated anti-Human CD3E. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Human CD3e/CD3 epsilon Flow Cytometry (FC) 5262
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Caption:
Flow cytometric analysis of Human CD3E expression on human whole blood lymphocytes. Cells were stained with APC-conjugated anti-Human CD3E. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Other CD3E Antibody Products
CD3e/CD3 epsilon 研究背景

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

 CD3e/CD3 epsilon 參考資料
  • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
  • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
  • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
  • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.
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