Anti-CD31 Antibody

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Anti-CD31 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-CD31 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Not React with: Mouse, Rat
Specificity
Human CD31
Immunogen
Recombinant Human CD31 protein (Catalog#10148-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD31 / PECAM1 (rh CD31 / PECAM1; Catalog#10148-H08H; EAW94208.1; Met 1-Lys 601). CD31 / PECAM1 specific IgG was purified by Human CD31 / PECAM1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD31 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:2500-1:10000
IP 0.5-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD31 Antibody (Rabbit Polyclonal antibody) Images

Immunochemical Staining of human CD31 in human stomach with Rabbit Polyclonal Antibody (1:5000, formalin-fixed paraffin embedded sections). Positive staining was localized to vascular endothelial cells.
Immunochemical Staining of human CD31 in human heart with Rabbit Polyclonal Antibody (1:5000, formalin-fixed paraffin embedded sections). Positive staining was localized to vascular endothelial cells.
Immunochemical Staining of human CD31 in human breast with Rabbit Polyclonal Antibody (1:5000, formalin-fixed paraffin embedded sections). Positive staining was localized to vascular endothelial cells.

Anti-CD31 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:83 kDa

Observed band size:110 kDa

(We are unsure as to the identity of these extra bands.)

CD31 was immunoprecipitated using:

Lane A:0.5 mg THP-1 Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-CD31 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD31 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 130 kDa

Observed band size: 130 kDa

Anti-CD31 Antibody: Alternative Names

Anti-CD31 Antibody; Anti-CD31/EndoCAM Antibody; Anti-endoCAM Antibody; Anti-GPIIA' Antibody; Anti-PECA1 Antibody; Anti-PECAM-1 Antibody

CD31 Background Information

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 13-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
Full Name
platelet/endothelial cell adhesion molecule 1
References
  • Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
  • Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.
  • 2, 5-Dihydro-2, 5-dimethoxyfuran crosslinked silk-chitosan blend tubular construct for vascular graft application
    Author
    Pawar, HS;Francis, NK;Rameshbabu, AP;Dhara, S;
    Year
    2016
    Journal
    Materials Today Communications
    Application
    Cell differentiation assay

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