CD19 Protein, Human, Recombinant (His Tag)

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CD19 Protein, Human, Recombinant (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD19 (Cat:11880-H08H)at 0.02μg/ml(100μL/well) can bind anti-human CD19 Mab with a linear range of 0.9-500 ng/ml.
Protein Construction
A DNA sequence encoding the human CD19 (P15391) extracellular domain (Met 1-Lys 291) was expressed, with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Pro 20
Molecule Mass
The recombinant human CD19 consists of 283 amino acids and predictes a molecular mass of 31.6 kDa. In SDS-PAGE under reducing conditions, rhCD19 migrates as an approximately 47 kDa band due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD19 Protein, Human, Recombinant (His Tag): Images

Measured by its binding ability in a functional ELISA. Immobilized human CD19 (Cat:11880-H08H)at 0.02μg/ml(100μL/well) can bind anti-human CD19 Mab with a linear range of 0.9-500 ng/ml.

CD19 Protein, Human, Recombinant (His Tag): Alternative Names

B4 Protein, Human; CVID3 Protein, Human

CD19 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell coreceptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.
Full Name
CD19 molecule
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.
  • Chimeric antigen receptors that trigger phagocytosis
    Author
    Morrissey, M;Williamson, A;Steinbach, A;Roberts, E;Kern, N;Headley, M;Vale, R;
    Year
    2018
    Journal
    bioRxiv
    Application
    Bead engulfment assay
  • Generation and characterization of avian-derived anti-human CD19 single chain fragment antibodies
    Author
    Tsai, KC;Chiang, CW;Lo, YN;Chang, FL;Lin, TY;Chang, CY;Chen, WC;Lee, YC;
    Year
    2018
    Journal
    Anim. Biotechnol.
    Application
    immunization
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