CD155 / PVR Protein, Human, Recombinant (Fc Tag)

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CD155 / PVR Protein, Human, Recombinant (Fc Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA
. Immobilized human DNAM1 at 2 μg/ml (100 μl/well) can bind human CD155-Fc with a linear ranger of 0.032-0.8 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Asn 343) of human CD155 (NP_006496.3) was expressed with the C-terminal fused Fc region of human IgG1.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Trp 21
Molecule Mass
The recombinant human CD155/Fc is a disulfide-linked homodimer. The reduced monomer consists of 561 amino acids and predicts a molecular mass of 61.8 kDa. As a result of glycosylation, the rhCD155/Fc monomer migrates as approximately 95-105 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD155 / PVR Protein, Human, Recombinant (Fc Tag): Images

CD155 / PVR Protein, Human, Recombinant (Fc Tag): Alternative Names

CD155 Protein, Human; HVED Protein, Human; Necl-5 Protein, Human; NECL5 Protein, Human; PVS Protein, Human; TAGE4 Protein, Human

CD155 / PVR Background Information

CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
Full Name
poliovirus receptor
References
  • Freistadt MS, et al. (2000) Hematopoietic cells from CD155-transgenic mice express CD155 and support poliovirus replication ex vivo. Microb Pathog. 29(4): 203-12.
  • Sato T, et al. (2004) Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions. Genes Cells. 9(9): 791-9.
  • Kakunaga S, et al. (2004) Enhancement of serum- and platelet-derived growth factor-induced cell proliferation by Necl-5/Tage4/poliovirus receptor/CD155 through the Ras-Raf-MEK-ERK signaling. J Biol Chem. 279(35): 36419-25.
  • Sato T, et al. (2005) Common signaling pathway is used by the trans-interaction of Necl-5/Tage4/PVR/CD155 and nectin, and of nectin and nectin during the formation of cell-cell adhesion. Cancer Sci. 96(9): 578-89.
  • Minami Y, et al. (2007) Involvement of up-regulated Necl-5/Tage4/PVR/CD155 in the loss of contact inhibition in transformed NIH3T3 cells. Biochem Biophys Res Commun. 352(4): 856-60.
  • CD226 Involves in Immune Synapse
    Author
    Wei, H;Sun, R;Tian, Z;Hou, S;Ge, K;Zheng, X;
    Year
    2014
    Journal
    J. Biol. Chem.
    Application
    binding
  • CD226 Protein Is Involved in Immune Synapse Formation and Triggers Natural Killer (NK) Cell Activation via Its First Extracellular Domain
    Author
    S Hou, K Ge, X Zheng, H Wei, R Sun, Z Tian
    Year
    2014
    Journal
    JOURNAL OF BIOLOGICAL CHEMISTRY
    Application
    binding
  • The receptors CD96 and CD226 oppose each other in the regulation of natural killer cell functions
    Author
    CJ Chan, L Martinet, S Gilfillan…
    Year
    2014
    Journal
    Nature immunology
  • TIGIT expression levels on CD4+ T cells are correlated with disease severity in patients with psoriasis
    Author
    Wang, FF;Wang, Y;Wang, L;Wang, TS;Bai, YP;
    Year
    2018
    Journal
    Clin. Exp. Dermatol.
    Application
    cell-based
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