|IP||4-6 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CD147 was immunoprecipitated using:
Lane A:0.5 mg K562 Whole Cell Lysate2 µL anti-CD147 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CD147 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 42 kDa
Observed band size: 50 kDa
Anti-CD147 rabbit monoclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:42 kDa
Observed band size:54 kDa
CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CD147 Antibody (PE)||Mouse||Monoclonal||FCM||10186-MM08-P|
|Anti-CD147 Antibody (FITC)||Rabbit||Monoclonal||FCM,ICC/IF,IF||10186-R125-F|
|Anti-CD147 Antibody (PE)||Rabbit||Monoclonal||FCM||10186-R125-P|
|Anti-CD147 Antibody (APC)||Mouse||Monoclonal||FCM||10186-MM08-A|
|Anti-CD147 Antibody (FITC)||Mouse||Monoclonal||FCM,ICC/IF,IF||10186-MM08-F|
|Anti-CD147 Antibody (APC)||Rabbit||Monoclonal||FCM||10186-R125-A|