CD146 / MCAM Protein, Human, Recombinant (Fc Tag)

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CD146 / MCAM Protein, Human, Recombinant (Fc Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Gly 559) of human CD146 precursor (NP_006491.2) was expressed with the C-terminal fused Fc region of human IgG1.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Val 24
Molecule Mass
The recombinant CD146/Fc chimera comprises 774 amino acids and predicts a molecular mass of 86.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 120-130 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD146 / MCAM Protein, Human, Recombinant (Fc Tag): Images

CD146 / MCAM Protein, Human, Recombinant (Fc Tag): Alternative Names

CD146 Protein, Human; MUC18 Protein, Human

CD146 / MCAM Background Information

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.
Full Name
melanoma cell adhesion molecule
References
  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
  • Wnt5a uses CD146 as a receptor to regulate cell motility and convergent extension
    Author
    Ye, Z;Zhang, C;Tu, T;Sun, M;Liu, D;Lu, D;Feng, J;Yang, D;Liu, F;Yan, X;
    Year
    2013
    Journal
    Nat Commun
  • Monoclonal antibodies to human laminin α4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of α6β1 integrin and MCAM to α4-laminins
    Author
    Ishikawa, T;Wondimu, Z;Oikawa, Y;Ingerpuu, S;Virtanen, I;Patarroyo, M;
    Year
    2014
    Journal
    Matrix Biol.
    Application
    binding
  • CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development
    Author
    Tu, T;Zhang, C;Yan, H;Luo, Y;Kong, R;Wen, P;Ye, Z;Chen, J;Feng, J;Liu, F;Wu, JY;Yan, X;
    Year
    2015
    Journal
    Cell Res.
  • CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development
    Author
    Chen, J;Luo, Y;Hui, H;Cai, T;Huang, H;Yang, F;Feng, J;Zhang, J;Yan, X;
    Year
    2017
    Journal
    Proc. Natl. Acad. Sci. U.S.A.
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