Anti-CD146 / MCAM Antibody

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Anti-CD146 / MCAM Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-CD146 / MCAM Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human CD146 / MCAM
No cross-reactivity in ELISA with
Human CD66a
Human CD166
Human CD106
Human BCAM
Human CD226
Immunogen
Recombinant Human CD146 protein (Catalog#10115-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559) .
Source
Monoclonal Rabbit IgG Clone #012
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD146 / MCAM Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD146 / MCAM Antibody (Rabbit Monoclonal antibody) Images

Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing membrane staining of endothelial cells (red arrow), epithelial cells (green arrow). The left panel: 1:200. The right panel: 1:1000.
Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200).
Immunohistochemical staining of formalin fixed, paraffin-embedded human kidney showing membrane staining of endothelial cells (red arrow) (1:200).

Anti-CD146 rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:132 kDa

Observed band size:140 kDa

Anti-CD146 rabbit monoclonal antibody at 1:500 dilution

Lane A: CD146 konckout Hela Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 10 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:132 kDa

Observed band size: kDa

Anti-CD146 / MCAM Antibody: Alternative Names

Anti-CD146 Antibody; Anti-MUC18 Antibody

CD146 / MCAM Background Information

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.
Full Name
melanoma cell adhesion molecule
References
  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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