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Beta-Tubulin Loading Control 抗體, 鼠單抗

  • Beta-Tubulin Loading Control Antibody, Mouse Mab, Immunofluorescence
  • Beta-Tubulin Loading Control Antibody, Mouse MAb, Western blot
產品資料評論相關產品實驗方法
 Beta-Tubulin 抗體產品資訊
免疫原:A synthetic peptide corresponding to the N-terminus of the human beta-tubulin
Clone ID:05
Ig類型:Mouse IgG2b
濃度:
內毒素:
緩衝液:0.2 μm filtered solution in PBS
抗體製備方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 Beta-Tubulin Antibody WB Application Image
 Beta-Tubulin Antibody IF Application Image
Beta-Tubulin Loading Control Antibody, Mouse Mab, Immunofluorescence
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Caption:
Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.
Other Beta-Tubulin Antibody Products
Beta-Tubulin 研究背景

Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.

 Beta-Tubulin 參考資料
  • Williams RC Jr. et al., 1999. Anal Biochem. 275(2): 265-7.
  • Nogales E. et al., 1998. Nat Struct Biol. 5(6): 451-8.
  • Dutcher SK. 2001. Curr Opin Cell Biol. 13(1): 49-54.
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