Anti-BMP4 Antibody

Cat: 10609-MM02
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Anti-BMP4 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-BMP4 Antibody
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human BMP4
Immunogen
Recombinant Human BMP-4 protein
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgM Clone #02
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BMP4 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BMP4 Antibody (Mouse Monoclonal antibody) Images
Human BMP-4/BMP-2B Immunofluorescence(IF) 2998
Immunofluorescence staining of Human BMP4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human BMP4 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human BMP-4/BMP-2B Flow Cytometry (FC) 5333
Flow cytometric analysis of Human BMP4 on HeLa cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human BMP4, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-BMP4 Antibody Alternative Names
Anti-BMP2B Antibody;Anti-BMP2B1 Antibody;Anti-MCOPS6 Antibody;Anti-OFC11 Antibody;Anti-ZYME Antibody
BMP4 Background Information
Full Name
bone morphogenetic protein 4
Product Description Host Clonality Application Catalog# (PDF)
Anti-BMP4 Antibody (PE) Mouse Monoclonal FCM 10609-MM02-P
info info
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