Antibody Purification

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Antibody Purification Technologies & Methods


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Antibody Purification

Antibody purification is a process in which antibodies are extracted from an antiserum which contains a mixture of compounds. Once purified, the antibodies can be used in a variety of ways. This process can be performed at labs which offer an antibody purification service, and people can also purify their own, if they have the necessary equipment and tools. This may be done in a lab where people want rapid turnarounds for their research, or when researchers are concerned about protecting the integrity of original research.

One of the major sources of antibodies is antiserum produced in animals. In this case, an animal is repeatedly injected with an antigen until it develops antibodies, and its blood is used to prepare an antiserum which can be purified to access the antibodies. Another source of antibodies is a cloned cell which produces antibodies as part of a colony of identical cells; this method is used for mass production of so-called "monoclonal antibodies."

There are several techniques which can be used for antibody purification. The most suitable technique depends on the medium being purified. Antibodies made in eggs, for example, are treated differently than antiserum from a rabbit or goat. Affinity chromatography and protein A/G are both used in the pursuit of antibody purification, for the purpose of isolating antibodies from the rest of the solution. Classically antibody purification relies on using a substance which binds to a specific antibody, holding in place while the rest of the solution is washed away.


Sweden, University Researcher:Here is the data about the Ago2 protein I got from you. I am very glad to say that it is highly active, performs very good in in vitro assays and is also stable at 4C for at least for 2 weeks when tested its enzymatic activit